rabbit polyclonal 3025s Search Results


pges2  (Bioss)
94
Bioss pges2
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Pges2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal 3025s  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit polyclonal 3025s
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Rabbit Polyclonal 3025s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3025s  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 3025s
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
3025s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3025s/product/Cell Signaling Technology Inc
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polyclonal anti arts antibody  (ProSci Incorporated)
94
ProSci Incorporated polyclonal anti arts antibody
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Polyclonal Anti Arts Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pkcδ  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti pkcδ
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Anti Pkcδ, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti irs1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti irs1
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Anti Irs1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β tubulin  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc β tubulin
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
β Tubulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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perk antibody  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc perk antibody
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Perk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pa1-1057  (Thermo Fisher)
90
Thermo Fisher pa1-1057
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Pa1 1057, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pirs1 y612  (Thermo Fisher)
90
Thermo Fisher pirs1 y612
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Pirs1 Y612, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ihc mcherry thermofisher ma  (Thermo Fisher)
86
Thermo Fisher ihc mcherry thermofisher ma
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
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phdac5 s259  (Millipore)
90
Millipore phdac5 s259
(A) Western blotting of <t>PGES2</t> protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.
Phdac5 S259, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Western blotting of PGES2 protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.

Journal: PLoS Biology

Article Title: Ablating astrocyte insulin receptors leads to delayed puberty and hypogonadism in mice

doi: 10.1371/journal.pbio.3000189

Figure Lengend Snippet: (A) Western blotting of PGES2 protein expression from FACS-isolated astrocytes were imaged and quantified for IR loxp and IRKO GFAP mice. IR loxp (black bar) and IRKO GFAP (white bar), ( n = 4 per group, 2 brains pooled per lane). Values are expressed as means ± SEM. * P < 0.05 IRKO GFAP versus IR loxp group. The underlying data can be found in . (B–C) Schematic diagram representing the mechanism of astrocyte modulation of HPG axis. FACS, fluorescence-activated cell sorting; HPG, hypothalamic pituitary gonadotropin; IR, insulin receptor; IRKO GFAP , astrocyte-specific insulin receptor deletion; PGES2, prostaglandin E synthase 2.

Article Snippet: The primary antibodies used were as follows: IRβ (Cat. #3025S, Cell signaling); PGES2 (Cat. #bs-2639R, Bioss) [ , ]; and GADPH (Cat.# SC-32233, Santa Cruz Biotechnology).

Techniques: Western Blot, Expressing, Isolation, Fluorescence, FACS